Objective: To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.
Methods: Genomic DNA was isolated using the AxyPrep DNA Kit, double-digested by two restrictional endonucleases (Eco RI and Mse I) and ligated to oligonucleotide adapters. After Pre-amplification and selective amplification, the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.
Results: More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.
Conclusion: The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.