Abstract
Transcriptional analysis using a DNA microarray is an extremely efficient method for analyzing two-component signal transduction networks. Here we introduce three such networks in Escherichia coli that were clarified using a DNA microarray: a PhoQ/PhoP system that senses extracellular Mg2+ and controls the gene expression for adaptation to environmental Mg2+ deprivation (Mg2+ stimulon); an EvgS/EvgA signal transduction system that activates expression of multiple drug efflux pumps and acid resistance genes; and a signal transduction cascade between EvgS/EvgA and PhoQ/PhoP.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Bacterial Physiological Phenomena*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Escherichia coli / genetics
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Escherichia coli / physiology
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Genes, Bacterial
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Magnesium / metabolism
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Metabolic Networks and Pathways
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Models, Biological
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Protein Kinases / genetics
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Protein Kinases / metabolism
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Signal Transduction / physiology*
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Bacterial Proteins
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Escherichia coli Proteins
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EvgA protein, E coli
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EvgS protein, E coli
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PhoP protein, E coli
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PhoQ protein, Bacteria
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Transcription Factors
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Protein Kinases
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Magnesium