Strategies for shotgun identification of integral membrane proteins by tandem mass spectrometry

Proteomics. 2008 Oct;8(19):3947-55. doi: 10.1002/pmic.200800120.

Abstract

Integral membrane proteins (IMPs) are difficult to identify, mainly for two reasons: the hydrophobicity of IMPs and their low abundance. Sample preparation is a key component in the large-scale identification of IMPs. In this review, we survey strategies for shotgun identification of IMPs by MS/MS. We will discuss enrichment, solubilization, separation, and digestion of IMPs, and data analysis for membrane proteomics.

Publication types

  • Review

MeSH terms

  • Animals
  • Humans
  • Membrane Proteins / analysis*
  • Membrane Proteins / isolation & purification
  • Proteomics / methods*
  • Tandem Mass Spectrometry / methods*

Substances

  • Membrane Proteins