We examined the prevalence of vitamin D deficiency and insufficiency in children attending our tuberculosis (TB) clinic during a 2-year period. Sixty-four patients were included with active TB (n = 26) or latent TB infection (n = 38). Eighty-six percent (n = 55) were either vitamin D deficient (serum 25-hydroxyvitamin D <20 nmol/L) or insufficient (serum 25-hydroxyvitamin D <75 nmol/L). Only 1 child with active TB was vitamin D replete.