Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR

O Frolow; B Endeward; O Schiemann; T F Prisner; J W Engels

doi:10.1093/nass/nrn078

Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR

Nucleic Acids Symp Ser (Oxf). 2008:(52):153-4. doi: 10.1093/nass/nrn078.

Authors

O Frolow¹, B Endeward, O Schiemann, T F Prisner, J W Engels

Affiliation

¹ Institut für Organische Chemie und Chemische Biologie (OCCB), Goethe-Universität Frankfurt am Main, Max-von-Laue Str. 7, D-60438 Frankfurt am Main, Germany.

PMID: 18776299
DOI: 10.1093/nass/nrn078

Abstract

Long range distance measurement on RNA allow the determination of RNA folds. Here we report the site specific incorporation of nitroxide spin labels at U,C and A by "on column synthesis". PELDOR (Pulsed Electron Double Resonance) measurements of several RNAs in the range of 2-6 nm were successful.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cyclic N-Oxides / chemistry*
DNA / chemical synthesis
Electron Spin Resonance Spectroscopy / methods
Iodine / chemistry
Oligonucleotides / chemical synthesis*
Oligonucleotides / chemistry
RNA / chemical synthesis
RNA / chemistry*
Spin Labels*

Substances

Cyclic N-Oxides
Oligonucleotides
Spin Labels
RNA
DNA
Iodine