Principles of resonance energy transfer

Curr Protoc Cytom. 2006 Nov:Chapter 1:Unit1.12. doi: 10.1002/0471142956.cy0112s38.

Abstract

This unit describes the basic principles of the fluorescence resonance energy (FRET) process. In addition, it characterizes available parameters and instruments for FRET measurements, discusses limitations, and shows a few examples of the application of FRET.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Energy Transfer
  • Fluorescence Polarization
  • Fluorescence Resonance Energy Transfer / instrumentation
  • Fluorescence Resonance Energy Transfer / methods
  • Fluorescence Resonance Energy Transfer / standards*
  • Luminescent Proteins
  • Microscopy, Fluorescence
  • Molecular Conformation
  • Software
  • Spectrometry, Fluorescence / methods*
  • Spectrometry, Fluorescence / standards

Substances

  • Luminescent Proteins