To better understand the impact of gender change in medical practice on health care in Tunisia, we conducted a cross-sectional study on the attitudes of 512 medical students (40% female) to medicine and medical practice in the Faculty of Medicine, Sousse. Female students attached more value than males to the intrinsic aspects of a physician's job, such as the desire to help others and to work with people. Regarding the dimensions of comprehensive care (psychosociological aspects of care, health education, patient-centred approach and medical prevention), there were wide differences between men and women. At the end of their training cycles, both male and female students ascribed less importance to these aspects than in the beginning.