Characterization of an extracellular polysaccharide produced by a Pseudomonas strain grown on glycerol

Bioresour Technol. 2009 Jan;100(2):859-65. doi: 10.1016/j.biortech.2008.07.002. Epub 2008 Aug 17.

Abstract

A new extracellular charged polysaccharide composed mainly by galactose, with lower amounts of mannose, glucose and rhamnose, was produced by the cultivation of Pseudomonas oleovorans NRRL B-14682 using glycerol as the sole carbon source. Thermal and solid-state NMR analysis showed that this polymer is essentially amorphous, with a glass transition temperature of 155.7 degrees C. The exopolysaccharide aqueous solutions have viscoelastic properties similar to that of Guar gum, but with affinity to salts as a result of its polyelectrolyte character. In addition, the exopolysaccharide has demonstrated good flocculating and emulsifying properties and film-forming capacity. These properties make this polymer a good alternative to more expensive natural polysaccharides, such as Guar gum, in several applications in the food, pharmaceutical, cosmetic, textile, paper and petroleum industries.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Proliferation
  • Elasticity
  • Gingiva / chemistry
  • Glycerol / metabolism*
  • Hardness
  • Polysaccharides, Bacterial / chemistry*
  • Polysaccharides, Bacterial / metabolism*
  • Pseudomonas oleovorans / chemistry*
  • Pseudomonas oleovorans / metabolism*
  • Species Specificity
  • Viscosity

Substances

  • Polysaccharides, Bacterial
  • Glycerol