Abstract
Improved methods for selective isolation of diverse actinomycetes of the genus Micromonospora and a genus-specific nested PCR for rapid identification of putative Micromonospora isolates were developed. The robustness of both the isolation and the identification approach was underpinned by phylogenetic analysis based on 16S rRNA gene sequences.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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China
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DNA Primers / genetics
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DNA, Bacterial / genetics
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Genes, Bacterial*
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Genes, rRNA
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Micromonospora / classification*
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Micromonospora / genetics*
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Micromonospora / isolation & purification
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Soil Microbiology*
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Species Specificity
Substances
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DNA Primers
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DNA, Bacterial
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RNA, Ribosomal, 16S