Effect of solvent on enantioselective esterification of naproxen by lipase with trimethylsilyl methanol

Biotechnol Bioeng. 1994 Jan 5;43(1):64-8. doi: 10.1002/bit.260430109.

Abstract

Improvement of stereoselective resolution of racemic Naproxen, 2-(6-methoxy-2-naphthyl)propionic acid, was attempted with esterifcation reaction by Candida cylindracea lipase. By carefully selecting the organic medium, a 72-time enhancement of yield of the desired S-ester was achieved. The optimal reaction temperature was approximately 53 degrees C, and an alcohol concentration between 20 mM and 40 mM in an 80% (v/v) isooctane and 20% (v/v) toluene mixture was found. (c) 1994 John Wiley & Sons, Inc.