Tristel Sporicidal Wipes are chlorine dioxide-based disinfectant wipes for disinfecting non-lumened semi-critical medical devices. In this study, the mycobactericidal activity of this product was assessed by a modified version of the European Standard prEN 14563 carrier test under clean conditions against Mycobacterium avium. The chlorine dioxide concentration in the activated wipe was 200ppm. The results showed that the chlorine dioxide wipes were mycobactericidal in 30s contact time with mechanical action and in 60s without mechanical action, both under clean conditions.