Abstract
A PCR assay has been developed based on a lolB (hemM) gene, which was found to be highly conserved among the Vibrio cholerae species but non-conserved among the other enteric bacteria. The lolB PCR detected all O1, O139 and non-O1/non-O139 serogroup and biotypes of V. cholerae. The analytical specificity of this assay was 100% while the analytical sensitivity was 10 pg/microL and 10(3) CFU/mL at DNA and bacterial level respectively. The diagnostic sensitivity and specificity was 98.5% and 100% respectively.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics*
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Coproporphyrinogen Oxidase / genetics*
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DNA, Bacterial / genetics
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Gram-Negative Bacteria / genetics
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Gram-Negative Bacteria / isolation & purification
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Gram-Negative Bacterial Infections / microbiology
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Humans
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Vibrio Infections / microbiology*
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Vibrio cholerae / genetics*
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Vibrio cholerae / isolation & purification*
Substances
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Bacterial Proteins
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DNA, Bacterial
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HemN protein, Bacteria
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Coproporphyrinogen Oxidase