Human Papillomavirus type distribution in invasive cervical cancer in Uganda

BMC Infect Dis. 2008 Jun 24:8:85. doi: 10.1186/1471-2334-8-85.

Abstract

Background: We conducted a study aiming to describe Human Papillomavirus (HPV) type distribution in invasive cervical carcinoma in Uganda.

Methods: 191 archival cervical carcinoma samples diagnosed in the Department of Pathology, Makerere University in Kampala between 1968 and 1992 were analysed using a sensitive PCR-Reverse Hybridization Line Probe Assay.

Results: Out of the 186 cases of confirmed invasive cervical cancer in the study paraffin blocks, 114 were positive for HPV DNA. Specific HPV genotypes were identifiable in 109 cases: HPV 16, 18, 31, 35, 39, 44, 45, 51, 52 and 70. These occurred as single infections in 105 cases (96.3%) and as multiple infections in 4 cases (3.7%). HPV 16 or 18 accounted for 80% (84/105) of cases with single infection.

Conclusion: The results of this study confirm the role of HPV 16 and 18 in cervical cancer pathogenesis in the Ugandan population. The results suggest that the currently available HPV vaccines against HPV 16 and 18 could possibly prevent the majority of invasive cervical cancers in Uganda.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma* / epidemiology
  • Adenocarcinoma* / virology
  • Carcinoma, Squamous Cell* / epidemiology
  • Carcinoma, Squamous Cell* / virology
  • Cervix Uteri / virology
  • DNA, Viral / analysis
  • DNA, Viral / isolation & purification
  • Female
  • Human papillomavirus 16 / isolation & purification
  • Human papillomavirus 18 / isolation & purification
  • Humans
  • Papillomaviridae / classification*
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / epidemiology
  • Papillomavirus Infections / virology
  • Uganda / epidemiology
  • Uterine Cervical Neoplasms* / epidemiology
  • Uterine Cervical Neoplasms* / virology

Substances

  • DNA, Viral