Abstract
Ribosomal protein S4 (RPSD), a part of the ribosomal small subunit, is one of the proteins that is a part of the ribosomal machinery and is a potential new target for the discovery of antibacterial agents. Continued screening of microbial extracts using antisense-sensitized rpsD Staphylococcus aureus strain led to the isolation of a new dimeric compound, phaeosphenone (2). Compound 2 showed broad-spectrum antibacterial activity against Gram-positive bacteria, exhibiting MIC values ranging from 8 to 64 microg/mL. Phaeosphenone showed the highest sensitivity for Streptococcus pneumoniae (8 microg/mL) and inhibited the growth of Candida albicans with an MIC of 8 microg/mL. Phaeosphenone showed a modest selectivity for the inhibition of RNA synthesis over DNA and protein synthesis in S. aureus.
MeSH terms
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Anthraquinones / chemistry
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Anthraquinones / isolation & purification*
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Anthraquinones / pharmacology*
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology*
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Antisense Elements (Genetics)
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Ascomycota / chemistry*
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Candida albicans / drug effects
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Gram-Positive Bacteria / drug effects
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Molecular Structure
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Nucleic Acid Synthesis Inhibitors / chemistry
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Nucleic Acid Synthesis Inhibitors / isolation & purification
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Nucleic Acid Synthesis Inhibitors / pharmacology
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Ribosomal Proteins / drug effects*
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Staphylococcus aureus / drug effects
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Streptococcus pneumoniae / drug effects
Substances
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Anthraquinones
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Anti-Bacterial Agents
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Antisense Elements (Genetics)
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Nucleic Acid Synthesis Inhibitors
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Ribosomal Proteins
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phaeosphenone
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ribosomal protein S4
Associated data
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GENBANK/AF181708
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GENBANK/AF196988
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GENBANK/AF817684