Candida albicans and three other Candida species contain an elongation factor structurally and functionally analogous to elongation factor 3

FEMS Microbiol Lett. 1991 May 1;64(1):45-9. doi: 10.1016/0378-1097(91)90207-q.

Abstract

A cell-free poly(U)-dependent translation elongation system from Candida albicans is ATP-dependent due to the presence of an elongation factor 3 (EF3)-like activity. Saccharomyces cerevisiae ribosomes added to a C. albicans postribosomal supernatant (PRS) supported poly(U)-dependent elongation, suggesting that the C. albicans lysate contained a soluble translation factor functionally analogous to the S. cerevisiae translation factor EF-3. The presence of EF-3 in C. albicans was confirmed by Western blotting using an antibody raised against S. cerevisiae EF-3. This antibody was also used to screen a selection of Candida species, all of which possessed EF-3 with molecular mass in the range of 110-130 kDa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida albicans / analysis*
  • Candida albicans / immunology
  • Fungal Proteins / chemistry
  • Fungal Proteins / isolation & purification*
  • Molecular Weight
  • Peptide Elongation Factors / chemistry
  • Peptide Elongation Factors / isolation & purification*
  • Peptide Elongation Factors / physiology
  • Protein Biosynthesis
  • Saccharomyces cerevisiae Proteins

Substances

  • Fungal Proteins
  • Peptide Elongation Factors
  • Saccharomyces cerevisiae Proteins
  • YEF3 protein, S cerevisiae