Human papillomavirus type 16 transcripts expressed from viral-cellular junctions and full-length viral copies in CaSki cells and in a cervical carcinoma

Virology. 1991 Jun;182(2):870-3. doi: 10.1016/0042-6822(91)90632-l.

Abstract

We have mapped using the RNA PCR the viral-cellular junctions of HPV16 viral-cellular cotranscripts expressed in CaSki cells and a cervical carcinoma to nt 3728 and 881, respectively. Both junctions were located within the E1-E2 region. Examination of the cellular sequences of the cotranscripts showed the presence of a polyadenylation signal in each of the transcripts. In CaSki cells and in the cervical carcinoma transcripts derived from the full-length early region including the E2 transcript were also detected. Our results suggest that the utilization of a cellular polyadenylation site could be important in the development of cancer by HPV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Carcinoma / genetics
  • Carcinoma / microbiology*
  • Cell Line
  • Female
  • Gene Expression Regulation, Viral*
  • Genes, Viral
  • Humans
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Papillomaviridae / genetics*
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • RNA Splicing
  • RNA, Messenger / genetics
  • RNA, Viral / genetics*
  • Uterine Cervical Neoplasms / genetics
  • Uterine Cervical Neoplasms / microbiology*
  • Viral Structural Proteins / genetics

Substances

  • Oligonucleotides
  • RNA, Messenger
  • RNA, Viral
  • Viral Structural Proteins