Isolation and biochemical characterization of a zona pellucida-binding glycoprotein of boar spermatozoa

FEBS Lett. 1991 Mar 11;280(1):183-6. doi: 10.1016/0014-5793(91)80233-s.

Abstract

Lectin-like molecules on the sperm surface are implicated in the process of gamete recognition and adhesion. We have isolated and biochemically characterized a 15 kDa glycoprotein from ejaculated boar sperm which possess zona pellucida-binding- and haemagglutinating-activity. The zona/15 kDa protein interaction is inhibited by fucoidan, suggesting that the glycoprotein is one of the sperm components which participate in the initial gamete interaction. N-Terminal sequence analysis of the isolated 15 kDa glycoprotein showed that it may belong to the same sperm/egg recognition-mediating protein family as the sea urchin sperm protein binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biological Evolution
  • Glycoproteins / chemistry*
  • Glycoproteins / genetics
  • Glycoproteins / metabolism
  • Male
  • Molecular Sequence Data
  • Molecular Weight
  • Polysaccharides / pharmacology
  • Receptors, Cell Surface / chemistry*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / metabolism
  • Sea Urchins
  • Spermatozoa / chemistry*
  • Spermatozoa / drug effects
  • Swine
  • Zona Pellucida / chemistry*
  • Zona Pellucida / drug effects

Substances

  • Glycoproteins
  • Polysaccharides
  • Receptors, Cell Surface
  • egg surface sperm receptor
  • fucoidan