Ingestion without inactivation of bacteriophages by Tetrahymena

J Eukaryot Microbiol. 2008 May-Jun;55(3):207-13. doi: 10.1111/j.1550-7408.2008.00316.x.

Abstract

Tetrahymena has been shown to ingest and inactivate bacteriophages, such as T4, in co-incubation experiments. In this study, Tetrahymena thermophila failed to inactivate phages PhiX174 and MS2 in co-incubations, although PhiX174 were ingested by T. thermophila, as demonstrated by: (1) recovery at defecation in a pulse-chase experiment, (2) recovery from Tetrahymena by detergent lysis, and (3) transmission electron microscopy. We conclude, therefore, that the phages must be digestion-resistant. Internalized PhiX174 were further shown to be partially protected from lethal damage by ultraviolet (UV) C and UVB irradiation. Finally, ingested PhiX174 were shown to be rapidly transported through buffer in a horizontal swimming, race tube-like assay. The transport and protection of phages may confer evolutionary advantages that explain the acquisition of digestion-resistance by some phages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteriophages / physiology*
  • Bacteriophages / radiation effects
  • Tetrahymena thermophila / physiology*
  • Tetrahymena thermophila / radiation effects
  • Tetrahymena thermophila / ultrastructure
  • Tetrahymena thermophila / virology
  • Ultraviolet Rays
  • Virus Inactivation