Analysis of galanthamine-type alkaloids by capillary gas chromatography-mass spectrometry in plants

Phytochem Anal. 2008 Jul-Aug;19(4):285-93. doi: 10.1002/pca.1028.

Abstract

Galanthamine, an acetylcholinesterase inhibitor used for the treatment of Alzheimer's disease, and galanthamine-type alkaloids are synthesised in different plants of the family Amaryllidaceae. A capillary gas chromatographic-mass spectroscopic (CGC-MS) method for the separation of 7 galanthamine type alkaloids, including galanthamine and epigalanthamine, is described in the present paper. A simple method for the routine quantification of galanthamine in plants was developed using pre-packed columns with diatomaceous earth (Isolute HM-N), allowing simultaneous preparation of a large number of samples. Galanthamine showed excellent linearity in the range from 50 to 1000 microg/mL and the limit of quantification was 5 microg/mL in total ion current mode and 1.6 ng/mL in selected ion monitoring mode. The recovery of galanthamine was more than 90%. Interday reproducibility (RSD) of the extraction was 2.74%. A method to find and to microextract Amaryllidaceae alkaloids in low-mass plant samples is also described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / analysis*
  • Alkaloids / isolation & purification
  • Calibration
  • Galantamine / analysis*
  • Galantamine / isolation & purification
  • Gas Chromatography-Mass Spectrometry / methods*
  • Plants / chemistry*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Alkaloids
  • Galantamine