Assays for the mannan-binding lectin pathway

Curr Protoc Immunol. 2004 Feb:Chapter 13:Unit 13.6. doi: 10.1002/0471142735.im1306s58.

Abstract

This unit contains protocols that can be used to measure mannan-binding lectin (MBL) levels and MBL pathway activity in human plasma or serum. Using a modification of the conventional ELISA, the detection reagent used in the methods described (e.g., an antibody or streptavidin) is labeled with Eu3+ instead of an enzyme. The sensitizing reagent used in these assays employ the use of anti-MBL antibody or mannan. The unit also includes an MBL/MASP complex activity assay to evaluate MBL activity in serum or plasma samples, which allows for the measurement of the specific activity of the MBL pathway.

Publication types

  • Review

MeSH terms

  • Complement Pathway, Mannose-Binding Lectin / physiology*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Humans
  • Mannose-Binding Lectin / analysis*
  • Mannose-Binding Lectin / blood
  • Mannose-Binding Lectin / immunology
  • Mannose-Binding Protein-Associated Serine Proteases / analysis*
  • Mannose-Binding Protein-Associated Serine Proteases / immunology
  • Mannose-Binding Protein-Associated Serine Proteases / metabolism

Substances

  • Mannose-Binding Lectin
  • Mannose-Binding Protein-Associated Serine Proteases