Assessment of the antibacterial activity of phenylethanoid glycosides from Phlomis lanceolata against multiple-drug-resistant strains of Staphylococcus aureus

J Nat Med. 2008 Jan;62(1):91-5. doi: 10.1007/s11418-007-0194-z. Epub 2007 Sep 26.

Abstract

Three phenylethanoid glycosides, forsythoside B (1), phlinoside C (2) and verbascoside (3), were isolated from the methanol extract of the leaves of Phlomis lanceolata, an Iranian medicinal plant, by reversed-phase preparative high-performance liquid chromatography (HPLC), and the structures of these compounds were elucidated conclusively by ultraviolet (UV), mass spectrometry (MS) and a series of 1D and 2D nuclear magnetic resonance (NMR) analyses. The antibacterial properties of 1-3 against five multi-drug-resistant (MDR) strains of Staphylococcus aureus have been assessed by the rapid and robust microtitre-plate-based serial dilution method. While compounds 1 and 3 showed considerable activities against all five strains, compound 2 was inactive at the test concentrations.

MeSH terms

  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / pharmacology*
  • Chromatography, High Pressure Liquid
  • Drug Resistance, Multiple, Bacterial*
  • Glucosides / pharmacology
  • Glycosides / chemistry
  • Glycosides / isolation & purification
  • Glycosides / pharmacology*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Microbial Sensitivity Tests
  • Molecular Structure
  • Phenols / pharmacology
  • Phlomis* / chemistry
  • Plant Leaves
  • Spectrophotometry, Ultraviolet
  • Staphylococcus aureus / drug effects*
  • Staphylococcus aureus / growth & development

Substances

  • Anti-Bacterial Agents
  • Glucosides
  • Glycosides
  • Phenols
  • phlinoside C
  • acteoside
  • forsythiaside