Abstract
In this study, the UVA (photo)protective activity of the phenolic fraction of L. caerulea fruits (PFLC) was assessed in human keratinocytes HaCaT. The keratinocytes were pre- or post-treated with PFLC (1-250 mg/l) and exposed to UVA irradiation (10-30 J/cm(2)). The results showed that both pre- and post-treatment with PFLC significantly suppressed UVA-induced ROS production, which was also revealed as a decrease in intracellular lipid peroxidation and elevation of reduced glutathione. Protection was concentration-dependent with a maximum at 50 mg/l. These results suggest that PFLC attenuates UVA-induced oxidative stress by reduction of ROS generation and ROS-mediated damage. For this reason, PFLC has potentially skin-protective functions against the deleterious effects of sunlight.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line, Transformed
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Free Radical Scavengers / metabolism
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Glutathione / drug effects
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Glutathione / metabolism
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Glutathione / radiation effects
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Humans
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Keratinocytes / drug effects*
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Keratinocytes / pathology
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Keratinocytes / radiation effects
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Lipid Peroxidation / drug effects
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Lipid Peroxidation / radiation effects
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Lonicera*
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Oxidative Stress / drug effects
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Oxidative Stress / radiation effects
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Phenol / chemistry
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Phytotherapy
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Plant Extracts / chemical synthesis
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Plant Extracts / pharmacology*
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Radiation Injuries / prevention & control*
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Reactive Oxygen Species / antagonists & inhibitors
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Reactive Oxygen Species / radiation effects
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Skin / drug effects*
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Skin / pathology
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Skin / radiation effects
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Ultraviolet Rays / adverse effects
Substances
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Free Radical Scavengers
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Plant Extracts
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Reactive Oxygen Species
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Phenol
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Glutathione