Abstract
mAtNOS1 is a novel gene recently reported in mammalian cells with functions that are not fully understood. The present study generated human neuroblastoma SHSY cells over- and underexpressing mAtNOS1 and shows that mAtNOS1 is involved in regulating mitochondrial nitric oxide, mitochondrial transmembrane potential, protein tyrosine nitration, cytochrome c release, and apoptosis of those cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Apoptosis Regulatory Proteins / genetics
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Apoptosis Regulatory Proteins / metabolism*
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Apoptosis* / genetics
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Arabidopsis / genetics
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Arabidopsis / metabolism
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Brain / embryology
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Brain / metabolism
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COS Cells
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Cell Line, Tumor
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Chlorocebus aethiops
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Embryo, Mammalian / embryology
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Embryo, Mammalian / metabolism
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GTP Phosphohydrolases
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GTP-Binding Proteins / genetics
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GTP-Binding Proteins / metabolism*
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Humans
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Membrane Potentials / genetics
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Mice
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Mitochondria / genetics
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Mitochondria / metabolism*
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Mitochondrial Membranes / metabolism
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Mutation
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism*
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Neuroblastoma / genetics
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Neuroblastoma / metabolism*
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Nitric Oxide / metabolism*
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Nitric Oxide Synthase / genetics
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Nitric Oxide Synthase / metabolism
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Sequence Homology, Amino Acid
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Thymus Gland / embryology
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Thymus Gland / metabolism
Substances
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Apoptosis Regulatory Proteins
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Arabidopsis Proteins
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Neoplasm Proteins
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Nitric Oxide
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NOA1 protein, Arabidopsis
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Nitric Oxide Synthase
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GTP Phosphohydrolases
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GTP-Binding Proteins
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NOA1 protein, human