Molecular evaluation of apoptotic versus antiapoptotic angiogenic markers in hepatocellular carcinoma

Clin Biochem. 2008 Aug;41(12):1008-14. doi: 10.1016/j.clinbiochem.2008.02.005. Epub 2008 Feb 21.

Abstract

Objective: To assess the role of HO-1 in HCC progression and to study the expression of apoptotic factors represented by TNF-alpha, and Fas-L versus antiapoptotic and angiogenic factors represented by HO-1, TGF-beta, HGF, and VEGF in HCC compared to non cancerous cirrhotic liver.

Design and methods: Liver biopsies were taken from twelve patients with grade II HCC confined to the liver and twelve patients with non cancerous liver cirrhosis (served as control). RT-PCR of previous genes was evaluated.

Results: HO-1, VEGF, HGF, and TNF-alpha genes were significantly increased (P<0.05) in HCC compared to control. Fas-L showed a significant decrease (P<0.05) in HCC compared to control. TGF-beta was higher in HCC than control but the difference was not statistically significant (P>0.05). HGF showed significant positive correlation with HO-1 (r=0.8217, P=0.001).

Conclusion: HCC is associated with increased expression of VEGF, HGF, and TGF-beta, and with suppression of Fas-L. In addition, HO-1 is highly significantly expressed in HCC. The significant positive correlation between HO-1 and HGF was first reported in Egyptian human liver biopsies, and this suggests that it may play a role in the progression of hepatocellular carcinoma.

MeSH terms

  • Adult
  • Angiogenic Proteins / biosynthesis*
  • Angiogenic Proteins / genetics
  • Apoptosis Regulatory Proteins / biosynthesis*
  • Apoptosis Regulatory Proteins / genetics
  • Biopsy
  • Carcinoma, Hepatocellular / genetics
  • Carcinoma, Hepatocellular / metabolism*
  • Carcinoma, Hepatocellular / pathology
  • Cytokines / genetics
  • Electrophoresis, Agar Gel
  • Heme Oxygenase-1 / biosynthesis*
  • Humans
  • Liver Cirrhosis / genetics
  • Liver Cirrhosis / metabolism
  • Liver Cirrhosis / pathology
  • Liver Neoplasms / genetics
  • Liver Neoplasms / metabolism*
  • Liver Neoplasms / pathology
  • Male
  • Middle Aged
  • RNA / genetics
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Angiogenic Proteins
  • Apoptosis Regulatory Proteins
  • Cytokines
  • RNA
  • Heme Oxygenase-1