Nucleotide insertion and bypass synthesis of pyrene- and BODIPY-modified oligonucleotides by DNA polymerases

Claudia Wanninger-Weiss; Francesca Di Pasquale; Thomas Ehrenschwender; Andreas Marx; Hans-Achim Wagenknecht

doi:10.1039/b718002k

Nucleotide insertion and bypass synthesis of pyrene- and BODIPY-modified oligonucleotides by DNA polymerases

Chem Commun (Camb). 2008 Mar 28:(12):1443-5. doi: 10.1039/b718002k. Epub 2008 Jan 29.

Authors

Claudia Wanninger-Weiss¹, Francesca Di Pasquale, Thomas Ehrenschwender, Andreas Marx, Hans-Achim Wagenknecht

Affiliation

¹ University of Regensburg, Institute for Organic Chemistry, D-93040 Regensburg, Germany.

PMID: 18338050
DOI: 10.1039/b718002k

Abstract

The chromophores pyrene and bordipyrromethenylbenzene directly linked to the 5-position of uridine are tolerated and recognized as thymine derivatives by DNA polymerases in primer extension experiments.

MeSH terms

Base Sequence
Boron Compounds / chemistry*
Catalysis
DNA-Directed DNA Polymerase / chemistry*
Molecular Sequence Data
Molecular Structure
Oligonucleotides / chemical synthesis*
Oligonucleotides / chemistry
Pyrenes / chemistry*
Spectrometry, Fluorescence / methods
Uridine / chemistry*

Substances

4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Boron Compounds
Oligonucleotides
Pyrenes
pyrene
DNA-Directed DNA Polymerase
Uridine