Effect of cleft palate repair on the susceptibility to contraction-induced injury of single permeabilized muscle fibers from congenitally-clefted goat palates

Cleft Palate Craniofac J. 2008 Mar;45(2):113-20. doi: 10.1597/06-171.1.

Abstract

Objective: Despite cleft palate repair, velopharyngeal competence is not achieved in approximately 15% of patients, often necessitating secondary surgical correction. Velopharyngeal competence postrepair may require the conversion of levator veli palatini muscle fibers from injury-susceptible type 2 fibers to injury-resistant type 1 fibers. As an initial step to determining the validity of this theory, we tested the hypothesis that, in most cases, repair induces the transformation to type 1 fibers, thus diminishing susceptibility to injury.

Interventions: Single permeabilized levator veli palatini muscle fibers were obtained from normal palates and nonrepaired congenitally-clefted palates of young (2 months old) and adult (14 to 15 months old) goats and from repaired palates of adult goats (8 months old). Repair was done at 2 months of age using a modified von Langenbeck technique.

Main outcome measures: Fiber type was determined by contractile properties and susceptibility to injury was assessed by force deficit, the decrease in maximum force following a lengthening contraction protocol expressed as a percentage of initial force.

Results: For normal palates and cleft palates of young goats, the majority of the fibers were type 2 with force deficits of approximately 40%. Following repair, 80% of the fibers were type 1 with force deficits of 20% +/- 2%; these deficits were 45% of those for nonrepaired cleft palates of adult goats (p < .0001).

Conclusion: The decrease in the percentage of type 2 fibers and susceptibility to injury may be important for the development of a functional levator veli palatini muscle postrepair.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Biomechanical Phenomena
  • Cleft Palate / pathology
  • Cleft Palate / surgery*
  • Disease Models, Animal
  • Goats
  • Muscle Contraction / physiology
  • Muscle Development / physiology
  • Muscle Fibers, Fast-Twitch / pathology*
  • Muscle Fibers, Fast-Twitch / physiology
  • Muscle Fibers, Slow-Twitch / pathology*
  • Muscle Fibers, Slow-Twitch / physiology
  • Palatal Muscles / growth & development
  • Palatal Muscles / pathology*
  • Palate / pathology
  • Palate, Hard / surgery
  • Palate, Soft / surgery
  • Sarcomeres / pathology
  • Sarcomeres / physiology
  • Stress, Mechanical
  • Time Factors