Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer

Ross Stevenson; Helen C Baxter; Alastair Aitken; Tom Brown; Robert L Baxter

doi:10.1016/j.bioorg.2007.12.005

Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer

Bioorg Chem. 2008 Oct;36(5):215-9. doi: 10.1016/j.bioorg.2007.12.005. Epub 2008 Feb 14.

Authors

Ross Stevenson¹, Helen C Baxter, Alastair Aitken, Tom Brown, Robert L Baxter

Affiliation

¹ School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh, Scotland EH9 3JJ, UK.

PMID: 18279908
DOI: 10.1016/j.bioorg.2007.12.005

Abstract

A synthetic library of ca. 10(13) single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3gamma. A single aptamer, which showed similar affinities (K(D) approximately 10(-8)M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.

MeSH terms

14-3-3 Proteins / cerebrospinal fluid
14-3-3 Proteins / genetics
14-3-3 Proteins / metabolism*
Animals
Aptamers, Nucleotide / metabolism*
Base Sequence
Chromatography, Affinity
Cloning, Molecular
DNA / genetics
DNA / metabolism
DNA, Single-Stranded / chemistry*
DNA, Single-Stranded / metabolism*
Humans
Molecular Sequence Data
Oligonucleotides / chemistry
Oligonucleotides / metabolism
Protein Binding
Recombinant Proteins / genetics
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
SELEX Aptamer Technique
Scrapie / transmission
Sheep

Substances

14-3-3 Proteins
Aptamers, Nucleotide
DNA, Single-Stranded
Oligonucleotides
Recombinant Proteins
DNA