Immunological studies with the venom of the scorpion Tityus serrulatus

Braz J Med Biol Res. 1991;24(2):171-80.

Abstract

1. We describe a "sandwich" enzyme-linked immunosorbent assay (ELISA) sensitive to quantities of scorpion (Tityus serrulatus) venom (TsV) in the range of 1-3 ng/ml sample. 2. Cross-reactivity with the venom from the rattlesnake Crotalus durissus terrificus and with venoms from several snakes of the Bothrops genus was detected only at concentrations higher than 1 microgram/ml sample. 3. A conventional ELISA is also described for the detection of antibodies against TsV. 4. Analysis by Western Blot (WB) demonstrated a 25-kDa protein band common to TsV and to the venoms of Bothrops moojeni, B. jararacussu and B. jararaca. 5. Venom from C. d. terrificus exhibited WB cross-reactive bands of 16 and 25 kDa with TsV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Chromatography, Affinity
  • Cross Reactions
  • Crotalid Venoms / immunology
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Feasibility Studies
  • Male
  • Rabbits
  • Scorpion Venoms / analysis*
  • Scorpion Venoms / immunology

Substances

  • Crotalid Venoms
  • Scorpion Venoms