Flow chamber analysis of size effects in the adhesion of spherical particles

Int J Nanomedicine. 2007;2(4):689-96.

Abstract

The non-specific adhesion of spherical micro- and nano-particles to a cell substrate is investigated in a parallel plate flow chamber. Differently from prior in-vitro analyses, the total volume of the particles injected into the flow chamber is kept fixed whilst the particle diameter is changed in the range 0.5-10 microm. It is shown that: (i) the absolute number of particles adherent to the cell layer per unit surface decreases with the size of the particle as d(-1.7); (ii) the volume of the particles adherent per unit surface increases with the size of the particles as d(+1.3). From these results and considering solely non-specific particles, the following hypothesis are generated (i) use the smallest possible particles in biomedical imaging and (ii) use the largest possible particles in drug delivery.

MeSH terms

  • Adhesiveness
  • Cell Adhesion / physiology*
  • Cells, Cultured
  • Computer Simulation
  • Endothelial Cells / physiology*
  • Flow Injection Analysis / methods*
  • Humans
  • Microspheres*
  • Models, Biological*
  • Particle Size
  • Shear Strength