Abstract
Polyclonal and monoclonal antibodies directed against Cryptosporidium oocysts or sporozoites were developed to identify and characterize sporozoite pellicle and apical complex antigens. A very large glycoprotein of Cryptosporidium sporozoites was identified by three monoclonal antibodies that also reacted with intracellular merozoites. The glycoprotein was also identified by polyclonal antibodies that were affinity-purified on nitrocellulose-bound recombinant proteins expressed by four lambda gtll genomic clones.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Blotting, Western
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Cryptosporidium / chemistry*
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Cryptosporidium / immunology
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Female
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Genomic Library
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Glycoproteins / immunology
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Glycoproteins / isolation & purification*
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Humans
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Mice
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Mice, Inbred BALB C
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Protozoan Proteins / immunology
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Protozoan Proteins / isolation & purification*
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Spores / chemistry
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Spores / immunology
Substances
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Antibodies, Monoclonal
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Glycoproteins
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Protozoan Proteins