2'-O-hydroxyalkoxymethylribonucleosides and their incorporation into oligoribonucleotides

Nucleosides Nucleotides Nucleic Acids. 2007;26(10-12):1509-12. doi: 10.1080/15257770701543007.

Abstract

A simple and efficient method for the preparation of pyrimidine 2'-O-hydroxyethoxymethylribonucleosides and 2'-O-hydroxypropoxymethylribonucleosides has been developed. These modified nucleosides were incorporated into oligoribonucleotides, which were shown to form stable RNA/RNA duplexes. The effect of 2' -O-modification in the antisense and sense strands of small interference RNA was evaluated in multi-drug resistant NIH 3T3 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Oligoribonucleotides / chemistry*
  • Pyrimidines / chemistry*
  • RNA / chemistry*
  • Ribonucleosides / chemical synthesis*
  • Ribonucleosides / chemistry

Substances

  • Oligoribonucleotides
  • Pyrimidines
  • Ribonucleosides
  • RNA
  • pyrimidine