Electric impedance assisted micropipette aspiration

Katrin Kovanen; Mikko Lukkari; Sami Purmonen; Timo Ylikomi; Jouko Viitanen; Pasi J Kallio

doi:10.1109/IEMBS.2007.4353671

Electric impedance assisted micropipette aspiration

Annu Int Conf IEEE Eng Med Biol Soc. 2007:2007:5823-6. doi: 10.1109/IEMBS.2007.4353671.

Authors

Katrin Kovanen¹, Mikko Lukkari, Sami Purmonen, Timo Ylikomi, Jouko Viitanen, Pasi J Kallio

Affiliation

¹ Institute of Automation and Control, Tampere University of Technology, 33101 Tampere, Finland.

PMID: 18003337
DOI: 10.1109/IEMBS.2007.4353671

Abstract

Micropipette aspiration is a technique to selectively isolate cells from cell cultures using suction pressure. Cells can selectively be isolated one by one from neighboring cells into the micropipette. This paper presents a novel micropipette aspiration system assisted by an impedance measurement system. Furthermore, a method to reduce the adhesion force at a single cell level for a gentler detachment of the cell from a cultivation surface and surrounding cell connections is proposed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Physiological Phenomena*
Cell Separation / methods*
Cells, Cultured / cytology*
Cells, Cultured / physiology*
Electric Impedance
Electrochemistry / methods*
Humans
Micromanipulation / methods*
Suction / methods*