Wash-free in-situ self-desalting and peptide enrichment by block copolymer analyzed with MALDI-TOFMS

Chem Commun (Camb). 2007 Nov 21:(43):4468-70. doi: 10.1039/b710741b. Epub 2007 Aug 28.

Abstract

A novel technique of simultaneous peptide enrichment and wash-free in-situ self-desalting for MALDI analysis is reported, where a newly synthesized block copolymer with a microphase-separated configuration is applied to embed salts with its hydrophilic domain of poly(ethylene oxide) and concentrate peptides with its hydrophobic domain of polysulfone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bicarbonates / chemistry
  • Cholic Acids / chemistry
  • Myoglobin / chemistry*
  • Peptides / chemistry*
  • Polyethylene Glycols / chemistry*
  • Sodium Dodecyl Sulfate / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Sulfones / chemistry*

Substances

  • Bicarbonates
  • Cholic Acids
  • Myoglobin
  • Peptides
  • Sulfones
  • poly(ethylene oxide)-polysulfone block copolymer
  • Sodium Dodecyl Sulfate
  • Polyethylene Glycols
  • 3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate