Automated extended volume imaging of tissue using confocal and optical microscopy

Conf Proc IEEE Eng Med Biol Soc. 2006:2006:133-6. doi: 10.1109/IEMBS.2006.259723.

Abstract

Conventional histologic techniques cannot readily be used for 3D reconstruction of large tissue volumes. We have developed an imaging rig which supports both confocal and light microscopy, and utilizes a surface imaging approach to serially image embedded tissue blocks while maintaining alignment and registration of the image series.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anatomy, Cross-Sectional / instrumentation*
  • Anatomy, Cross-Sectional / methods
  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Image Enhancement / methods
  • Image Interpretation, Computer-Assisted / instrumentation
  • Image Interpretation, Computer-Assisted / methods
  • Imaging, Three-Dimensional / instrumentation*
  • Imaging, Three-Dimensional / methods
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Confocal / methods
  • Microtomy / instrumentation*
  • Microtomy / methods
  • Robotics / instrumentation*
  • Robotics / methods
  • Specimen Handling / instrumentation*
  • Specimen Handling / methods
  • Subtraction Technique / instrumentation