Genetic polymorphism in collagen type 1 alpha 2 intron 33 can be used for the initial screening of East Asians quickly and easily by a heteroduplex analysis

Leg Med (Tokyo). 2008 Mar;10(2):88-91. doi: 10.1016/j.legalmed.2007.07.004. Epub 2007 Sep 25.

Abstract

To save time and simplify the matching genetic information of bereaved family with the collected samples for identification of individuals in a mass-scale disaster and terrorism using molecular biological methods, we propose a method to initially screen samples according to different populations. We found a unique deletion (26822-3) in an allele that 62.0% of East Asian individuals had in intron 33 of type I collagen alpha 2 (COL1A2), while most Europeans and Africans did not have it. By making a heteroduplex complex, a visual discrimination was possible using 10% PAGE. A PCR and heteroduplex analysis could identify genetic polymorphism of COL1A2 intron 33 both clearly and accurately, even from decomposed samples or very small amounts of samples within a very short time period (approximately within 150min). As a result, genetic polymorphism of COL1A2 intron 33 is thus considered to be useful to quickly and easily screen East Asians from vast number of samples using a heteroduplex analysis. Consequently, we consider that an initial screening method for East Asians which use a heteroduplex analysis of genetic polymorphism of COL1A2 intron 33 can smoothly and effectively perform various identifications among East Asians when supplies are logistically poor after a mass-scale disaster or terrorism.

MeSH terms

  • Asia, Eastern / ethnology
  • Collagen / genetics*
  • Collagen Type I
  • Forensic Anthropology / methods
  • Genetic Testing / methods*
  • Heteroduplex Analysis*
  • Humans
  • Introns / genetics*
  • Polymorphism, Genetic / genetics*

Substances

  • Collagen Type I
  • Collagen