Spatial and temporal regulation of the forisome gene for1 in the phloem during plant development

Plant Mol Biol. 2007 Oct;65(3):285-94. doi: 10.1007/s11103-007-9217-0. Epub 2007 Aug 11.

Abstract

Forisomes are protein aggregates found uniquely in the sieve elements of Fabaceaen plants. Upon wounding they undergo a reversible, calcium-dependent conformational switch which enables them to act as cellular stopcocks. Forisomes begin to form in young sieve elements at an early stage of metaphloem differentiation. Genes encoding forisome components could therefore be useful as markers of early sieve element development. Here we present a comprehensive analysis of the developmental expression profile of for1, which encodes such a forisome component. The for1 gene is highly conserved among Fabaceaen species and appears to be unique to this phylogenetic lineage since no orthologous genes have been found in other plants, including Arabidopsis and rice. Even so, transgenic tobacco plants expressing reporter genes under the control of the for1 promoter display reporter activity exclusively in immature sieve elements. This suggests that the regulation of sieve element development is highly conserved even in plants where mature forisomes have not been detected. The promoter system could therefore provide a powerful tool for the detailed analysis of differentiation in metaphloem sieve elements in an unexpectedly broad range of plant species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fabaceae / genetics
  • Fabaceae / growth & development
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Developmental*
  • Gene Expression Regulation, Plant*
  • Glucuronidase / genetics
  • Glucuronidase / metabolism
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Microscopy, Confocal
  • Phloem / genetics*
  • Phloem / growth & development
  • Phloem / metabolism
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plants, Genetically Modified
  • Promoter Regions, Genetic / genetics
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Plant Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • Glucuronidase