The use of visible absorption spectra to evaluate different color reactions for the detection of cyclic ureides

Pharmazie. 2007 Jun;62(6):416-8.

Abstract

Modifications of the Zwikker- and Parri color detection tests were investigated and compared according to their ability to distinguish between nine different barbituric acids and hydantoins. Solutions of the resulting complexes in 50% DMSO were analyzed spectrophotometrically. 350 spectra have been analyzed and criteria for their assessment have been defined. The evaluation based upon the occurrence of a peak in the visible absorption spectra, in comparison with the spectrum of the blank solution. The results were in accordance to those obtained in the visual assessment using a color palette formerly introduced. Cobalt(II) nitrate and methanolic solution of piperidine, or cyclohexylamine, respectively, were the suitable components to get unmistakable results.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amines / chemistry
  • Color
  • Colorimetry
  • Dimethyl Sulfoxide
  • Indicators and Reagents
  • Reproducibility of Results
  • Spectrophotometry
  • Urea / analogs & derivatives*
  • Urea / analysis*

Substances

  • Amines
  • Indicators and Reagents
  • Urea
  • Dimethyl Sulfoxide