Testing of diagnostic methods for detection of influenza virus for optimal performance in the context of an influenza surveillance network

J Clin Microbiol. 2007 Sep;45(9):3109-10. doi: 10.1128/JCM.00697-07. Epub 2007 Jul 25.

Abstract

Influenza surveillance networks must detect early the viruses that will cause the forthcoming annual epidemics and isolate the strains for further characterization. We obtained the highest sensitivity (95.4%) with a diagnostic tool that combined a shell-vial assay and reverse transcription-PCR on cell culture supernatants at 48 h, and indeed, recovered the strain.

Publication types

  • Evaluation Study

MeSH terms

  • Humans
  • Influenza, Human / virology*
  • Molecular Diagnostic Techniques / methods*
  • Orthomyxoviridae / genetics
  • Orthomyxoviridae / growth & development
  • Orthomyxoviridae / isolation & purification*
  • Sensitivity and Specificity
  • Sentinel Surveillance
  • Virology / methods*
  • Virus Cultivation / methods*