Purification and characterization of beta-mannosidases from white rot fungus Phlebia radiata

Pol J Microbiol. 2007;56(2):139-47.

Abstract

A beta-mannosidase was purified from Phlebia radiata grown in a medium containing wheat bran or galactomannan as a carbon source. Maximal activity was observed at pH 5.5 and at 50 degrees C. Highly purified isoforms of beta-mannosidase (GM-1, GM-2) isolated from media containing galactomanan and (OT-1) media with wheat bran were obtained by means of column chromatography on Q-Sepharose, SP-Sepharose and chromatofocusing on Polybuffer Exchanger PBE-94.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basidiomycota / enzymology*
  • Carbon / metabolism
  • Chromatography, Liquid
  • Dietary Fiber / metabolism
  • Enzyme Stability
  • Galactose / analogs & derivatives
  • Hydrogen-Ion Concentration
  • Mannans / metabolism
  • Temperature
  • beta-Mannosidase / isolation & purification*
  • beta-Mannosidase / metabolism*

Substances

  • Dietary Fiber
  • Mannans
  • galactomannan
  • Carbon
  • beta-Mannosidase
  • Galactose