Crystal structure of the IL-15-IL-15Ralpha complex, a cytokine-receptor unit presented in trans

Nat Immunol. 2007 Sep;8(9):1001-7. doi: 10.1038/ni1492. Epub 2007 Jul 22.

Abstract

Interleukin 15 (IL-15) and IL-2, which promote the survival of memory CD8(+) T cells and regulatory T cells, respectively, bind receptor complexes that share beta- and gamma-signaling subunits. Receptor specificity is provided by unique, nonsignaling alpha-subunits. Whereas IL-2 receptor-alpha (IL-2Ralpha) is expressed together in cis with the beta- and gamma-subunits on T cells and B cells, IL-15Ralpha is expressed in trans on antigen-presenting cells. Here we present a 1.85-A crystal structure of the human IL-15-IL-15Ralpha complex. The structure provides insight into the molecular basis of the specificity of cytokine recognition and emphasizes the importance of water in generating this very high-affinity complex. Despite very low IL-15-IL-2 sequence homology and distinct receptor architecture, the topologies of the IL-15-IL-15Ralpha and IL-2-IL-2Ralpha complexes are very similar. Our data raise the possibility that IL-2, like IL-15, might be capable of being presented in trans in the context of its unique receptor alpha-chain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Crystallization
  • Humans
  • Interleukin-15 / chemistry*
  • Interleukin-15 / genetics
  • Interleukin-15 Receptor alpha Subunit / chemistry*
  • Interleukin-2 / chemistry
  • Interleukin-2 / genetics
  • Molecular Sequence Data
  • Protein Binding
  • Protein Structure, Quaternary*
  • Receptors, Interleukin-2 / chemistry
  • Receptors, Interleukin-2 / genetics
  • Sequence Homology, Amino Acid
  • Structural Homology, Protein

Substances

  • Interleukin-15
  • Interleukin-15 Receptor alpha Subunit
  • Interleukin-2
  • Receptors, Interleukin-2

Associated data

  • PDB/2Z3Q
  • PDB/2Z3R