Crystallization of and preliminary X-ray data for the negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa

J Mol Biol. 1991 Dec 20;222(4):869-71. doi: 10.1016/0022-2836(91)90579-u.

Abstract

The negative regulator (AmiC) of the amidase operon of Pseudomonas aeruginosa has been purified from an over-expressing clone and crystalized. Crystals of diffraction quality were obtained from polyethylene glycol 4000 and ammonium sulphate. AmiC crystallizes in P4(2)2(1)2 (a = 104.4 A, c = 66.6 A) with one subunit in the asymmetric unit. Crystals diffract beyond 2.8 A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidohydrolases / genetics*
  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / genetics
  • Genes, Bacterial
  • Genes, Regulator
  • Indicators and Reagents
  • Macromolecular Substances
  • Open Reading Frames
  • Operon*
  • Periplasmic Binding Proteins*
  • Protein Conformation
  • Pseudomonas aeruginosa / enzymology
  • Pseudomonas aeruginosa / genetics*
  • Repressor Proteins / chemistry
  • Restriction Mapping
  • X-Ray Diffraction / methods

Substances

  • Bacterial Proteins
  • Indicators and Reagents
  • Macromolecular Substances
  • Periplasmic Binding Proteins
  • Repressor Proteins
  • AmiC protein, Pseudomonas aeruginosa
  • Amidohydrolases