Comparative study of high-resolution microimaging with 30-MHz scanner for evaluating cardiac function in mice

J Am Soc Echocardiogr. 2007 Oct;20(10):1203-10. doi: 10.1016/j.echo.2007.02.026. Epub 2007 Jun 25.

Abstract

Background: The accurate assessment of cardiac function in mice is challenging because of their small heart size and rapid heart rate.

Methods: We examined the usefulness of novel high-resolution echocardiography (HRE) with a 30-MHz transducer in evaluating cardiac function in 20 mice compared with conventional echocardiography (CE) with a 13-MHz transducer. The left ventricular (LV) regional wall motion (RWM), LV end-diastolic dimension, fractional shortening, anterior LV wall thickness, E/A, and myocardial performance index were assessed.

Results: RWM analysis was more feasible by HRE than by CE (P < .05). Interobserver agreement in RWM analysis and correlation in LV end-diastolic dimension, fractional shortening, anterior LV wall thickness, E/A, and myocardial performance index were all better with HRE than CE.

Conclusions: HRE is superior to CE in assessing LV function in mice. HRE is potentially a useful method for accurate assessment of cardiac function in various mice models.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Diastole
  • Disease Models, Animal
  • Echocardiography / methods*
  • Heart Ventricles / diagnostic imaging*
  • Heart Ventricles / physiopathology
  • Image Enhancement / methods*
  • Male
  • Mice
  • Myocardial Contraction / physiology*
  • Myocardial Infarction / complications
  • Myocardial Infarction / diagnostic imaging
  • Myocardial Infarction / physiopathology
  • Observer Variation
  • Reproducibility of Results
  • Severity of Illness Index
  • Ventricular Dysfunction, Left / diagnostic imaging
  • Ventricular Dysfunction, Left / etiology
  • Ventricular Dysfunction, Left / physiopathology*