Binary cell fate specification during C. elegans embryogenesis driven by reiterated reciprocal asymmetry of TCF POP-1 and its coactivator beta-catenin SYS-1

Development. 2007 Jul;134(14):2685-95. doi: 10.1242/dev.008268. Epub 2007 Jun 13.

Abstract

C. elegans embryos exhibit an invariant lineage comprised primarily of a stepwise binary diversification of anterior-posterior (A-P) blastomere identities. This binary cell fate specification requires input from both the Wnt and MAP kinase signaling pathways. The nuclear level of the TCF protein POP-1 is lowered in all posterior cells. We show here that the beta-catenin SYS-1 also exhibits reiterated asymmetry throughout multiple A-P divisions and that this asymmetry is reciprocal to that of POP-1. Furthermore, we show that SYS-1 functions as a coactivator for POP-1, and that the SYS-1-to-POP-1 ratio appears critical for both the anterior and posterior cell fates. A high ratio drives posterior cell fates, whereas a low ratio drives anterior cell fates. We show that the SYS-1 and POP-1 asymmetries are regulated independently, each by a subset of genes in the Wnt/MAP kinase pathways. We propose that two genetic pathways, one increasing SYS-1 and the other decreasing POP-1 levels, robustly elevate the SYS-1-to-POP-1 ratio in the posterior cell, thereby driving A-P differential cell fates.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Body Patterning
  • Caenorhabditis elegans / embryology
  • Caenorhabditis elegans / physiology*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / physiology*
  • Cell Differentiation
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • High Mobility Group Proteins / genetics
  • High Mobility Group Proteins / physiology*
  • MAP Kinase Signaling System
  • Mutation
  • TCF Transcription Factors / genetics
  • TCF Transcription Factors / physiology
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Wnt Proteins / physiology
  • beta Catenin / physiology*

Substances

  • Caenorhabditis elegans Proteins
  • DNA-Binding Proteins
  • High Mobility Group Proteins
  • Sys-1 protein, C elegans
  • TCF Transcription Factors
  • Transcription Factors
  • Wnt Proteins
  • beta Catenin
  • pop-1 protein, C elegans