Aim: To establish a model of human M5 leukemia and investigate the pathomorphological change in severe combined immunodeficient (SCID) mice after being transplanted with SHI-1 cells.
Methods: Various dose of SHI-1 cells were transplanted i.p. into SCID mice. Serial tail vein samples at regular intervals were prepared for detecting the expression of CD14, CD137L on SHI-1 cells by flow cytometry (FCM). All dying mice were dissectted and the liver, spleen, kidney and bone marrow were examined to detect the appearance and distribution of the SHI-1 cells by FCM and/or immunohistochemistry.
Results: Tumors appeared in the abdomenion of the mice treated with various dose of SHI-1 cells. However, the dissemination of SHI-1 cells in murine tissues was found only in the groups of middle/high dose. SHI-1 cells were firstly found in tail vein samples 3 weeks after transplantation, and the time of engraftment was related to the dose of cells.
Conclusion: The SHI-1/SCID mice was constructed, which provided a useful tool to investigate acute monocytic leukemia(AML).