Expansion of intensity correlation spectroscopy for lifetime measurements--application to intracellular oxygen dynamics measurements

J Biomed Opt. 2007 Mar-Apr;12(2):020503. doi: 10.1117/1.2717623.

Abstract

We report on a simple correlation method for lifetime measurements using a random modulated excitation light source. We use an intensity correlation function of emission for lifetime analyses. In this method, no reference timing of the excitation is required. We apply the correlation method to measure phosphorescence decays and successfully demonstrate in the analysis of the phosphorescence decay from Pd(II) porphine in HeLa cells under aerobic and anaerobic conditions to understand the oxygen dynamics in individual cells. The method is applicable to faster decay time measurements down to a nanosecond range when the detection system is improved. Current fluorescence correlation setups can easily be modified for lifetime measurements, expanding the applicability in biological problems.

Publication types

  • Evaluation Study

MeSH terms

  • Algorithms*
  • HeLa Cells
  • Humans
  • Oxygen / metabolism*
  • Oxygen Consumption / physiology*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Fluorescence / methods*

Substances

  • Oxygen