pH-dependent association of enolase and glyceraldehyde-3-phosphate dehydrogenase of Lactobacillus crispatus with the cell wall and lipoteichoic acids

J Bacteriol. 2007 Jun;189(12):4539-43. doi: 10.1128/JB.00378-07. Epub 2007 Apr 20.

Abstract

The plasminogen-binding proteins enolase and glyceraldehyde-3-phosphate dehydrogenase of Lactobacillus crispatus were localized on the cell surface at pH 5 but released into the medium at an alkaline pH. These proteins bound to lipoteichoic acids at a pH below their isoelectric point. The results indicate that lactobacilli rapidly modify their surface properties in response to changes in pH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Wall / enzymology*
  • Glyceraldehyde 3-Phosphate
  • Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) / metabolism*
  • Hydrogen-Ion Concentration
  • Lactobacillus / enzymology*
  • Lipopolysaccharides / metabolism
  • Phosphopyruvate Hydratase / metabolism*
  • Plasminogen / metabolism
  • Protein Binding
  • Protein Biosynthesis
  • Teichoic Acids / metabolism
  • Transcription, Genetic

Substances

  • Lipopolysaccharides
  • Teichoic Acids
  • Glyceraldehyde 3-Phosphate
  • lipoteichoic acid
  • Plasminogen
  • Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
  • Phosphopyruvate Hydratase