Abstract
The mechanisms at the origin of heterogeneous carbapenem resistance levels observed among Acinetobacter baumannii isolates collected in 2005 in a large University Hospital of Rome, Italy, were investigated. These isolates were related and possessed similar plasmids carrying the carbapenem-hydrolyzing oxacillinase gene bla(OXA-58) but showed variable levels of resistance to carbapenems. Analysis of sequences surrounding the bla(OXA-58) gene showed genetic variability, with the presence in several isolates of multiple copies of the bla(OXA-58) gene; this extra copy number was likely related to an IS26-mediated transposition or recombination process.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acinetobacter baumannii / drug effects*
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Acinetobacter baumannii / enzymology
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Acinetobacter baumannii / genetics*
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Acinetobacter baumannii / isolation & purification
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Anti-Bacterial Agents / pharmacology*
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Base Sequence
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Carbapenems / pharmacology*
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Cloning, Molecular
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DNA, Bacterial / analysis
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DNA, Bacterial / genetics
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Gene Dosage*
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Genes, Bacterial*
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Genetic Variation
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Humans
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Microbial Sensitivity Tests
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Nucleic Acid Amplification Techniques
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Plasmids
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Polymerase Chain Reaction
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Sequence Analysis, DNA
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beta-Lactam Resistance*
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beta-Lactamases / biosynthesis
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beta-Lactamases / genetics
Substances
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Anti-Bacterial Agents
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Carbapenems
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DNA, Bacterial
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beta-Lactamases
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oxacillinase