Self-assembly of medium-chain alkyl monoglucosides in ammonium sulfate solutions with poly(ethylene glycol)

Langmuir. 2007 May 8;23(10):5358-66. doi: 10.1021/la063427d. Epub 2007 Apr 13.

Abstract

We study the phase behavior and microstructure of alkyl-beta-monoglucosides with intermediate chain lengths (octyl- and nonyl-beta-glucoside) in aqueous solutions containing ammonium sulfate and poly(ethylene glycol) (PEG). When the glucoside surfactants are mixed with PEG of molecular weight 3350 or larger, two different phase transitions are observed in the temperature range 0-100 degrees C, with lower and upper miscibility gaps separated by a one-phase isotropic region. Isothermal titration calorimetry is used to quantify the effect of PEG on the micellization properties of the alkyl monoglucosides, whereas small-angle neutron scattering gives insight into the microstructure of the surfactant/polymer mixtures near the liquid-liquid phase boundary. Results show that the range and the strength of the interactions in these solutions are highly affected by the presence of PEG. Solutions with nonyl-beta-glucoside contain larger micelles than those with octyl-beta-glucoside, and the intermicellar interactions are much stronger and longer ranged. The relevance of these findings for membrane protein crystallization is discussed.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Ammonium Sulfate / chemistry*
  • Crystallization
  • Glucosides / chemistry*
  • Hot Temperature
  • Membrane Proteins / chemistry
  • Micelles*
  • Phase Transition*
  • Polyethylene Glycols / chemistry*

Substances

  • Glucosides
  • Membrane Proteins
  • Micelles
  • octyl-beta-D-glucoside
  • Polyethylene Glycols
  • Ammonium Sulfate