The JAM Test and its daughter P-JAM: simple tests of DNA fragmentation to measure cell death and stasis

Nat Protoc. 2006;1(2):672-82. doi: 10.1038/nprot.2006.107.

Abstract

Cytotoxic T lymphocytes, and other death-inducing agents, have at least two different ways of killing their targets: drilling holes in the target cell membrane, or triggering the targets to commit suicide. The JAM Test is a method that measures the DNA fragmentation that accompanies cell suicide. We label target cells with radioactive DNA-precursor nucleotides and harvest them onto fiberglass filters, which trap large pieces of DNA but pass smaller fragments of apoptotic cells. As a general measure of apoptosis, the JAM Test described here is faster (can be completed in 4 h [or less if labeling is done the night before]), more quantitative, easier, more sensitive, more flexible and cheaper than most other current assays of apoptosis. The P-JAM, also discussed, additionally allows for assessment of death in cells that don't fragment their DNA, and allows for assays of agents that induce cell stasis rather than death.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Cell Death
  • Cytotoxicity Tests, Immunologic / methods*
  • DNA Fragmentation*
  • Mice
  • Sensitivity and Specificity
  • Spleen / cytology
  • T-Lymphocytes, Cytotoxic / immunology*