Surveillance for reassortant virus by multiplex reverse transcription-PCR specific for eight genomic segments of avian influenza A H5N1 viruses

Prasert Auewarakul; Kantima Sangsiriwut; Kridsda Chaichoune; Arunee Thitithanyanont; Witthawat Wiriyarat; Taweesak Songserm; Rasameepen Ponak-nguen; Jarunee Prasertsopon; Phisanu Pooruk; Pathom Sawanpanyalert; Parntep Ratanakorn; Pilaipan Puthavathana

doi:10.1128/JCM.00382-07

Surveillance for reassortant virus by multiplex reverse transcription-PCR specific for eight genomic segments of avian influenza A H5N1 viruses

J Clin Microbiol. 2007 May;45(5):1637-9. doi: 10.1128/JCM.00382-07. Epub 2007 Mar 21.

Authors

Prasert Auewarakul¹, Kantima Sangsiriwut, Kridsda Chaichoune, Arunee Thitithanyanont, Witthawat Wiriyarat, Taweesak Songserm, Rasameepen Ponak-nguen, Jarunee Prasertsopon, Phisanu Pooruk, Pathom Sawanpanyalert, Parntep Ratanakorn, Pilaipan Puthavathana

Affiliation

¹ Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Prannok Road, Bangkok Noi, Bangkok 10700, Thailand.

Abstract

Avian influenza H5N1 virus is a global threat. An emergence of a reassortant virus with a pandemic potential is a major concern. Here we describe a multiplex reverse transcription-PCR assay that is specific for the eight genomic segments of the currently circulating H5N1 viruses to facilitate surveillance for a virus resulting from reassortment between human influenza virus and the H5N1 virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genome, Viral*
Humans
Influenza A Virus, H5N1 Subtype / genetics*
Influenza A Virus, H5N1 Subtype / isolation & purification*
Influenza, Human / epidemiology
Influenza, Human / virology
Population Surveillance / methods
Reassortant Viruses / genetics*
Reverse Transcriptase Polymerase Chain Reaction / methods*